MUC6 · Western blot design guide

MUC6 Western Blot Planning Guide

Plan a MUC6 Western blot around the catalog-observed 257.1 kDa band, image-backed A02143 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for MUC6 (MUC6): expected band 257.1 kDa, antibody A02143, and PMC-cited SDS-PAGE protocol steps — MUC6 Western blot protocol sheet — expected band 257.1 kDa, antibody A02143, controls and PMC citations. Open the full MUC6 WB guide →

Navigate to: Protocols Band Controls Antibodies

MUC6 Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	257.1 kDa
	Observed band	Not reported — verify product WB image
	Gel	4-12% gradient
	Positive control ⓘ	Stomach
	Negative control ⓘ	Adipose tissue

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	257.1 kDa
ⓘ	Localization	Secreted
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Human / Mouse

Section 1

Real Curated MUC6 Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Seminal vesicle
Gel %	4-12% gradient
Load	20-30 µg total protein per lane
Transfer	Wet/tank, extended transfer
Membrane	0.45 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	A02143 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 2

What Is the Expected MUC6 Western Blot Band Size?

Use the product-observed 257.1 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
257.1 kDa	Matches the authoritative product WB observation.
257.1 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected MUC6 appearancePlan around 257.1 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	257.1 kDa; use this as the primary experimental expectation.
Calculated mass	257.1 kDa from UniProt Q6W4X9; retain as context.
Gel selection	4-12% gradient; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with A02143.

Why is my band missing or off?
Situation	Likely cause	Next action
257.1 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt Q6W4X9 Human Protein Atlas product datasheet (A02143)

Experiment evidence

Western blot images showing MUC6 band

A02143 · MUC6

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Sample controls for MUC6 Western blot

🧪Use Stomach as the first positive-control candidate and Adipose tissue as the HPA Not detected negative candidate.

Positive control: Stomach (High)

Negative control: Adipose tissue (Not detected)

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for MUC6

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Stomach	Reported tissue cells	High	Protein (HPA)	HPA →
Seminal vesicle	Reported tissue cells	High	Protein (HPA)	HPA →
Gallbladder	Reported tissue cells	Medium	Protein (HPA)	HPA →
Pancreas	Reported tissue cells	Low	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source
Adipose tissue	Reported tissue cells	Not detected	Protein (HPA)	HPA →
Appendix	Reported tissue cells	Not detected	Protein (HPA)	HPA →

Section 3

Advanced MUC6 Western Blot Tips

Deeper troubleshooting and optimisation questions for MUC6, answered from its protein features.

Which band should guide the blot?

Use 257.1 kDa, the observation attached to the authoritative A02143 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 257.1 kDa expectation.

Which positive control should I start with?

Start with Stomach, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use Adipose tissue as an orthogonal HPA Not detected candidate.

Which gel should I use?

Use 4-12% gradient consistently across the quick facts, protocol table, and poster.

What transfer method to use for MUC6 Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should A02143 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC8542475, PMC12828932, PMC6959463.

Boster reagents

MUC6 Western Blot Reagents

Human/Mouse-reactive MUC6 Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for MUC6 using A02143; observed band 257.1 kDa

Anti-Gastric Mucin/MUC6 Antibody Picoband®

Cat # A02143

View product →

Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.

Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.