Anti-GATA3 Antibody

Product Name

Anti-GATA3 Antibody

View all GATA-3 Antibodies

SKU/Catalog Number

A00593

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GATA3 Antibody (Catalog # A00593). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

GATA3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-GATA3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00593)

Host

Rabbit

Contents

GATA3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

GATA3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GATA3. The immunogen is located within amino acids 340 - 390 of GATA3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Phospho-SAMHD1 antibody will only detect the phosphorylated form of SAMHD1.

Reactive Species

A00593 is reactive to GATA3 in Human, Mouse, Rat

Applications

A00593 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

47.916kDa

Background of GATA-3

GATA3 is a zinc finger transcription factor that was first recognized as a possible determinant of T cell development. Later studies demonstrated that it is expressed in trophoblast giant cells and regulates trophoblast-specific genes during development. Specifically, GATA3 is selectively expressed in the trophectoderm of the peri-implantation embryo and regulates CDX2, which is required for the transcriptional repression of the POU5F1/Oct4 and NANOG genes and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Decreased or null-expression expression of GATA3 has also been suggested to play a major role in the development and progression of luminal breast cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

GATA3 antibody can be used for detection of GATA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of GATA3 in K562 cell lysate with GATA3 antibody at 1 μg/mL.

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Immunohistochemistry of GATA3 in rat brain tissue with GATA3 antibody at 2.5 μg/mL.

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Immunofluorescence of GATA3 in Rat Brain tissue with GATA3 antibody at 20 μg/mL.

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Immunohistochemistry of GATA3 in mouse brain tissue with GATA3 antibody at 2.5 μg/ml.

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Immunofluorescence of GATA3 in mouse brain tissue with GATA3 Antibody at 20 μg/mL.

Gene/Protein Information For GATA3 (Source: Uniprot.org, NCBI)

Alternative Names

131320; GATA binding protein 3; GATA3; GATA-3; GATA-binding factor 3; GATA-binding protein 3; HDRMGC5199; MGC2346; MGC5445; trans-acting T-cell-specific transcription factor GATA-3 GATA3 HDR, HDRS GATA binding protein 3 trans-acting T-cell-specific transcription factor GATA-3|GATA-binding factor 3

For more info on GATA3, check out the GATA3 Infographic

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In this infographic, you will see the following information for GATA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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