Click image to see more details
Product Info Summary
| SKU: | A11011 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Guanylate-binding protein 4 GBP4 Antibody
SKU/Catalog Number
A11011
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Guanylate-binding protein 4 GBP4 Antibody catalog # A11011. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Guanylate-binding protein 4 GBP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11011)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
3A8
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GBP4. AA range:551-600
Reactive Species
A11011 is reactive to GBP4 in Human, Mouse
Calculated molecular weight
73.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11011 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of RAW cells using GBP4 Polyclonal Antibody
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GBP4 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Guanylate-binding protein 4 GBP4 Antibody (A11011)
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Customer Q&As
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7 Customer Q&As for Anti-Guanylate-binding protein 4 GBP4 Antibody
Question
See attached the WB image, lot number and protocol we used for placenta testis using anti-GBP4 antibody A11011. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-20
Question
My question regards to test anti-GBP4 antibody A11011 on human placenta testis for research purposes, then I may be interested in using anti-GBP4 antibody A11011 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The products we sell, including anti-GBP4 antibody A11011, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-08
Question
Would A11011 anti-GBP4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Thomas
Verified customer
Asked: 2018-03-22
Answer
You can see on the product datasheet, A11011 anti-GBP4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-22
Question
Is this A11011 anti-GBP4 antibody reactive to the isotypes of GBP4?
E. Miller
Verified customer
Asked: 2017-09-06
Answer
The immunogen of A11011 anti-GBP4 antibody is Synthesized peptide derived from the C-terminal region of human GBP4. at AA rangle: 520-600. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-06
Question
Would anti-GBP4 antibody A11011 work on horse WB with spleen?
T. Walker
Verified customer
Asked: 2017-08-07
Answer
Our lab technicians have not tested anti-GBP4 antibody A11011 on horse. You can run a BLAST between horse and the immunogen sequence of anti-GBP4 antibody A11011 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse spleen in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-07
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta testis using anti-GBP4 antibody A11011. Let me know if you need anything else.
G. Edwards
Verified customer
Asked: 2017-05-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-16
Question
We are currently using anti-GBP4 antibody A11011 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
M. Zhao
Verified customer
Asked: 2013-05-13
Answer
The anti-GBP4 antibody (A11011) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-13
