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Product Info Summary
| SKU: | M00281 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-GCSF Receptor (CD114) Monoclonal Antibody
SKU/Catalog Number
M00281
BM5283 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GCSF Receptor (CD114) Monoclonal Antibody catalog # M00281. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GCSF Receptor (CD114) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00281)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACFC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GCSF Receptor (CD114) Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.
Reactive Species
M00281 is reactive to CSF3R in Human
Observed Molecular Weight
92 kDa
Calculated molecular weight
92.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00281 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
FC 1:20
Positive Control
WB: Caco-2 cell
Validation Images & Assay Conditions
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Western blot analysis of GCSF Receptor (CD114) expression in Caco-2 cell lysate.
Specific Publications For Anti-GCSF Receptor (CD114) Monoclonal Antibody (M00281)
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Customer Q&As
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7 Customer Q&As for Anti-GCSF Receptor (CD114) Monoclonal Antibody
Question
Would M00281 anti-GCSF Receptor (CD114) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
You can see on the product datasheet, M00281 anti-GCSF Receptor (CD114) Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-08
Question
Is a blocking peptide available for product anti-GCSF Receptor (CD114) Monoclonal antibody (M00281)?
Verified Customer
Verified customer
Asked: 2019-04-15
Answer
We do provide the blocking peptide for product anti-GCSF Receptor (CD114) Monoclonal antibody (M00281). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-04-15
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-GCSF Receptor (CD114) Monoclonal antibody M00281. Let me know if you need anything else.
A. Johnson
Verified customer
Asked: 2018-10-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-11
Question
I see that the anti-GCSF Receptor (CD114) Monoclonal antibody M00281 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-07-05
Question
Does anti-GCSF Receptor (CD114) Monoclonal antibody M00281 work on dog WB with blood?
Verified Customer
Verified customer
Asked: 2017-11-21
Answer
Our lab technicians have not tested anti-GCSF Receptor (CD114) Monoclonal antibody M00281 on dog. You can run a BLAST between dog and the immunogen sequence of anti-GCSF Receptor (CD114) Monoclonal antibody M00281 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-21
Question
I was wanting to use your anti-GCSF Receptor (CD114) Monoclonal antibody for WB for human placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human placenta identification?
A. Bhatt
Verified customer
Asked: 2017-07-20
Answer
It shows on the product datasheet, M00281 anti-GCSF Receptor (CD114) Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-20
Question
I was wanting to use to test anti-GCSF Receptor (CD114) Monoclonal antibody M00281 on human placenta for research purposes, then I may be interested in using anti-GCSF Receptor (CD114) Monoclonal antibody M00281 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Gonzalez
Verified customer
Asked: 2014-03-14
Answer
The products we sell, including anti-GCSF Receptor (CD114) Monoclonal antibody M00281, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-03-14
