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Product Info Summary
| SKU: | M30972 |
|---|---|
| Size: | 100μl/vial |
| Reactive Species: | All species |
| Host: | Mouse |
| Application: | Dot blot, ELISA, WB |
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Product info
Product Name
Anti-GFP Tag Antibody (monoclonal, 8C1)
SKU/Catalog Number
M30972
Size
100μl/vial
Form
Liquid
Description
Boster Bio Anti-GFP Tag Antibody (monoclonal, 8C1) catalog # M30972. Tested in Dot blot, ELISA, WB applications. This antibody reacts with All species.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GFP Tag Antibody (monoclonal, 8C1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30972)
Host
Mouse
Contents
0.5mg/ml in PBS, pH7.4, containing 50% glycerol.
Clonality
Monoclonal
Clone Number
8C1
Isotype
IgG1
Immunogen
This antibody is produced by immunizing animals with a synthetic GFP tag peptide corresponding to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M30972 is reactive to Tmem177 in All species
Applications
M30972 is guaranteed for Dot blot, ELISA, WB Boster Guarantee
Observed Molecular Weight
45 kDa
Calculated molecular weight
34.067kDa
Background of Tmem177
Green fluorescent protein is a 27 Kd protein, derived from the bioluminescent jellyfish Aequorea Victoria. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence. Flourescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool where they function as a fluorescent protein tag. Many recombinant proteins have been engineered with GFP tags to facilitate the detection, isolation and purification of the proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
1:500-1:5000
Validation Images & Assay Conditions
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Western blot analysis of different concentration of Anti-GFP (8C1) Monoclonal Antibody
Antigen: Extracts from E.coli expressing GFP-tagged protein
Line No.
1: 1 ug/mL
2: 0.5 ug/mL
3: 0.1 ug/mL
Blocking: Incubate the membrane with 5% Non-Fat Milk, PBS, 4 ℃ overnight
Antibody Incubation: Diluted antibody in 5% Non-Fat Milk, TBS, 0.1% Tween-20, RT, 30mins
Protein Target Info & Infographic
Gene/Protein Information For Tmem177 (Source: Uniprot.org, NCBI)
Gene Name
Tmem177
Full Name
Transmembrane protein 177
Weight
34.067kDa
Superfamily
TMEM177 family
Alternative Names
MGC10993; transmembrane protein 177
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tmem177, check out the Tmem177 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tmem177: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GFP Tag Antibody (monoclonal, 8C1) (M30972)
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No publications found for M30972
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Customer Reviews
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Customer Q&As
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