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Product Info Summary
| SKU: | A04016-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-GGA2 Antibody Picoband®
SKU/Catalog Number
A04016-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GGA2 Antibody Picoband® catalog # A04016-2. Tested in ELISA, Flow Cytometry, IP, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GGA2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04016-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human GGA2 recombinant protein (Position: L26-A608). Human GGA2 shares 85.4% amino acid (aa) sequence identity with mouse GGA2.
Reactive Species
A04016-2 is reactive to GGA2 in Human
Observed Molecular Weight
70 kDa
Calculated molecular weight
67.2 kDa
Background of GGA2
ADP-ribosylation factor-binding protein GGA2 is a protein that in humans is encoded by the GGA2 gene. ADP-ribosylation factor-binding protein GGA2 is a protein that in humans is encoded by the GGA2 gene. This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04016-2 is guaranteed for ELISA, Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human THP-1 whole cell, human HepG2 whole cell, human 293T whole cell, human K562 whole cell
FCM: HepG2 cell
IP: HepG2 whole cell
Validation Images & Assay Conditions
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Western blot analysis of GGA2 using anti-GGA2 antibody (A04016-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGA2 antigen affinity purified polyclonal antibody (Catalog # A04016-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GGA2 at approximately 70 kDa. The expected band size for GGA2 is at 67 kDa.
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Flow Cytometry analysis of HepG2 cells using anti-GGA2 antibody (A04016-2).
Overlay histogram showing HepG2 cells stained with A04016-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GGA2 Antibody (A04016-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Immunoprecipitating GGA2 in HepG2 whole cell lysate.
Western blot analysis of GGA2 using anti-GGA2 antibody (A04016-2);
Lane 1: HepG2 whole cell lysates (30ug);
Lane 2: Rabbit control IgG instead of anti-GGA2 antibody in HepG2 whole cell lysate;
Lane 3: anti-GGA2 antibody (2μg) + HepG2 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GGA2 antigen affinity purified polyclonal antibody (A04016-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for GGA2 at approximately 70 kDa. The expected band size for GGA2 is at 67 kDa.
Specific Publications For Anti-GGA2 Antibody Picoband® (A04016-2)
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