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Product Info Summary
| SKU: | PB9706 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GJC2 Antibody Picoband®
SKU/Catalog Number
PB9706
PB0741 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GJC2 Antibody Picoband® catalog # PB9706. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GJC2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9706)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GJC2, different from the related mouse and rat sequences by five amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9706 is reactive to GJC2 in Human, Mouse, Rat
Observed Molecular Weight
47 kDa
Calculated molecular weight
47.0 kDa
Background of GJC2
Gap junction gamma-2 (GJC2), also known as connexin-46.6 (Cx46.6) and connexin-47 (Cx47) and gap junction alpha-12 (GJA12), is a protein that in humans is encoded by the GJC2 gene. It is mapped to 1q42.13. This gene encodes a gap junction protein. Gap junction proteins are members of a large family of homologous connexins and comprise 4 transmembrane, 2 extracellular, and 3 cytoplasmic domains. This gene plays a key role in central myelination and is involved in peripheral myelination in humans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9706 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, U20S Whole Cell, HELA Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of GJC2 using anti-GJC2 antibody (PB9706).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Mouse Brain Tissue Lysate at 50ug,
Lane 3: U20S Whole Cell Lysate at 40ug,
Lane 4: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJC2 antigen affinity purified polyclonal antibody (Catalog # PB9706) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJC2 at approximately 47 kDa. The expected band size for GJC2 is at 47 kDa.
Specific Publications For Anti-GJC2 Antibody Picoband® (PB9706)
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6 Customer Q&As for Anti-GJC2 Antibody Picoband®
Question
We are currently using anti-GJC2 antibody PB9706 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
The anti-GJC2 antibody (PB9706) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-08
Question
Would anti-GJC2 antibody PB9706 work for WB with brain chondrosarcoma?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
According to the expression profile of brain chondrosarcoma, GJC2 is highly expressed in brain chondrosarcoma. So, it is likely that anti-GJC2 antibody PB9706 will work for WB with brain chondrosarcoma.
Boster Scientific Support
Answered: 2020-05-05
Question
I was wanting to use your anti-GJC2 antibody for WB for rat brain chondrosarcoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain chondrosarcoma identification?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
As indicated on the product datasheet, PB9706 anti-GJC2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain chondrosarcoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-15
Question
Can you help my question with product PB9706, anti-GJC2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9706 anti-GJC2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-03
Question
Would anti-GJC2 antibody PB9706 work on dog WB with brain chondrosarcoma?
P. Taylor
Verified customer
Asked: 2018-07-31
Answer
Our lab technicians have not validated anti-GJC2 antibody PB9706 on dog. You can run a BLAST between dog and the immunogen sequence of anti-GJC2 antibody PB9706 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog brain chondrosarcoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-31
Question
Is a blocking peptide available for product anti-GJC2 antibody (PB9706)?
S. Miller
Verified customer
Asked: 2013-12-24
Answer
We do provide the blocking peptide for product anti-GJC2 antibody (PB9706). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-12-24
