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Product Info Summary
| SKU: | A07510 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-GLG1 Antibody (C-term)
SKU/Catalog Number
A07510
Size
80 µl
Description
Boster Bio Anti-GLG1 Antibody (C-term) (Catalog # A07510). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GLG1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07510)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This GLG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1152-1179 amino acids from the C-terminal region of human GLG1.
Reactive Species
A07510 is reactive to GLG1 in Human, Mouse
Calculated molecular weight
134.6 kDa
Background of GLG1
The enzyme 17-beta hydroxysteroid dehydrogenase-12 (HSD17B12) uses NADPH to reduce 3-ketoacyl-CoA to 3-hydroxyacyl-CoA during the second step of fatty acid elongation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07510 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:50-1:100
Validation Images & Assay Conditions
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All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: T47D whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 136 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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GLG1 Antibody (C-term) western blot analysis in HepG2,T47D cell line lysates (35ug/lane).This demonstrates the GLG1 antibody detected the GLG1 protein (arrow).
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Western blot analysis of GLG1 Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). GLG1 (arrow) was detected using the purified Pab.
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All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: mouse cerebellum lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: T47D whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 136 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution
Lane 1: MCF-7 lysate
Lane 2: HepG2 whole cell lysate
Lane 3: T47D whole cell lysate
Lane 4: mouse cerebellum lysate
Lane 5: human placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 136 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
GLG1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLG1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Specific Publications For Anti-GLG1 Antibody (C-term) (A07510)
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