Anti-GLG1 Antibody (C-term)

Product Name

Anti-GLG1 Antibody (C-term)

View all Golgi Glycoprotein 1/GLG1 Antibodies

SKU/Catalog Number

A07510

Size

80 µl

Description

Boster Bio Anti-GLG1 Antibody (C-term) (Catalog # A07510). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-GLG1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07510)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This GLG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1152-1179 amino acids from the C-terminal region of human GLG1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07510 is reactive to GLG1 in Human, Mouse

Applications

A07510 is guaranteed for IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

134.552kDa

Background of Golgi Glycoprotein 1/GLG1

The enzyme 17-beta hydroxysteroid dehydrogenase-12 (HSD17B12) uses NADPH to reduce 3-ketoacyl-CoA to 3-hydroxyacyl-CoA during the second step of fatty acid elongation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000
IHC-P: 1:50-1:100

Validation Images & Assay Conditions

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All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: T47D whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 136 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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GLG1 Antibody (C-term) western blot analysis in HepG2,T47D cell line lysates (35ug/lane).This demonstrates the GLG1 antibody detected the GLG1 protein (arrow).

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Western blot analysis of GLG1 Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). GLG1 (arrow) was detected using the purified Pab.

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All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: mouse cerebellum lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: T47D whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 136 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Click image to see more details

All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution
Lane 1: MCF-7 lysate
Lane 2: HepG2 whole cell lysate
Lane 3: T47D whole cell lysate
Lane 4: mouse cerebellum lysate
Lane 5: human placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 136 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Click image to see more details

GLG1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLG1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Gene/Protein Information For GLG1 (Source: Uniprot.org, NCBI)

Alternative Names

CFR1; CFR-1; cysteine-rich fibroblast growth factor receptor; DKFZp686L08213; E-selectin ligand 1; ESL1; ESL-1; FLJ23319; FLJ23967; FLJ32797; GLG1; Golgi apparatus protein 1; Golgi Glycoprotein 1; golgi sialoglycoprotein MG-160; MG160; MG-160 GLG1 CFR-1, ESL-1, MG-160, MG160 golgi glycoprotein 1 Golgi apparatus protein 1|E-selectin ligand 1|cysteine-rich fibroblast growth factor receptor|golgi sialoglycoprotein MG-160

For more info on GLG1, check out the GLG1 Infographic

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In this infographic, you will see the following information for GLG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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