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- Table of Contents
GLG1 antibody (A07510-1) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of SiHa cells using anti-GLG1 antibody (A07510-1).
Overlay histogram showing SiHa cells stained with A07510-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLG1 Antibody (A07510-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SiHa cells stained with A07510-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLG1 Antibody (A07510-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-GLG1 Antibody Picoband®
GLG1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
GLG1 antibody was positively detected by WB in:
human HepG2 whole cell, human SiHa whole cell, human Hacat whole cell, human A431 whole cell, rat testis tissue, mouse testis tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GLG1 antibody was positively detected by IHC in:
mouse kidney tissue, mouse kidney tissue, rat kidney tissue, rat kidney tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
GLG1 antibody was positively detected by FC in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-GLG1 Antibody Picoband®
View all GLG1 Antibodies
View all GLG1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human GLG1 recombinant protein (Position: E173-K1040). Human GLG1 shares 98.5% and 98% amino acid (aa) sequence identity with mouse and rat GLG1, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
150 kDa
Calculated Molecular Weight
134.6 kDa
Background of GLG1
Golgi apparatus protein 1 is a protein that in humans is encoded by the GLG1 gene. The GLG1 gene, also known as Golgi apparatus protein 1, encodes a protein involved in maintaining the structural integrity and function of the Golgi apparatus, a vital organelle involved in protein processing and trafficking within the cell. GLG1 is associated with the formation of Golgi stacks and the regulation of vesicular transport between the endoplasmic reticulum and Golgi complex. It plays a critical role in protein glycosylation, sorting, and secretion. Dysregulation of GLG1 expression or function has been implicated in various cellular processes and diseases, including cancer progression, neurodegenerative disorders, and congenital disorders of glycosylation. Understanding the molecular mechanisms underlying GLG1 function is essential for unraveling its role in cellular physiology and its potential as a therapeutic target for diseases associated with Golgi dysfunction.
Cite This Product
Anti-GLG1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07510-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
99%
High Homology
Mouse
99%
High Homology
Dog
99%
High Homology
Rat
98%
High Homology
Cow
98%
High Homology
Sheep
98%
High Homology
Zebrafish
81%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GLG1 antibody
Immunohistochemistry (IHC) protocol for GLG1 antibody
Flow Cytometry (FC) protocol for GLG1 antibody
Publications
Application:
Species:
Hypoxia inhibits pulmonary artery endothelial cell apoptosis via the e-selectin/biliverdin reductase pathway
Published: 2016-03-28
Journal: MICROVASCULAR RESEARCH
Application: IF,WB
Impact Factor: 2.30
Species: Rat
PMID: 27033411
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Product Promise
We promise Anti-GLG1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A07510-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026