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- Table of Contents
GLIPR2 antibody (A10232-1) performs well in ELISA, Flow Cytometry, IP, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of THP-1 cells using anti-GLIPR2 antibody (A10232-1).
Overlay histogram showing THP-1 cells stained with A10232-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLIPR2 Antibody (A10232-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with A10232-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLIPR2 Antibody (A10232-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-GLIPR2 Antibody Picoband®
GLIPR2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IP, IHC, WB |
Tested Applications
GLIPR2 antibody was positively detected by WB in:
human U251 whole cell, human U20S whole cell, human THP-1 whole cell, human Hela whole cell, rat brain tissue, rat RH35 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GLIPR2 antibody was positively detected by IHC in:
human lung cancer tissue, human lung cancer tissue, rat spleen tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
GLIPR2 antibody was positively detected by FC in:
THP-1 cell
GLIPR2 antibody was positively detected by IP in:
U251 whole cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Rat
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg /1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-GLIPR2 Antibody Picoband®
View all GLIPR2 Antibodies
View all GLIPR2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human GLIPR2 recombinant protein (Position: M1-K154). Human GLIPR2 shares 93.5% amino acid (aa) sequence identity with mouse GLIPR2.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
17 kDa
Calculated Molecular Weight
17.2 kDa
Background of GLIPR2
The GLIPR2 gene, also known as Golgi-associated plant pathogenesis-related protein 2, encodes a protein localized to the Golgi apparatus and involved in diverse cellular processes, including apoptosis, autophagy, and vesicular trafficking. GLIPR2 has been implicated in regulating cell growth, differentiation, and survival, with potential roles in cancer development and progression. Additionally, GLIPR2 expression has been linked to neurodegenerative disorders and inflammatory responses. Its precise molecular functions and downstream signaling pathways are still being elucidated, but it represents a promising target for therapeutic interventions in various diseases. Understanding the biological significance of GLIPR2 is essential for deciphering its role in cellular homeostasis and disease pathogenesis.
Cite This Product
Anti-GLIPR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10232-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
98%
High Homology
Rabbit
97%
High Homology
Cow
96%
High Homology
Sheep
96%
High Homology
Rat
94%
High Homology
Mouse
94%
High Homology
Zebrafish
73%
Moderate Homology
Drosophila
47%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GLIPR2 antibody
Immunohistochemistry (IHC) protocol for GLIPR2 antibody
Flow Cytometry (FC) protocol for GLIPR2 antibody
Immunoprecipitation (IP) protocol for GLIPR2 antibody
Publications
Application:
Species:
Identification of pivotal genes and regulatory networks associated with SAH based on multi-omics analysis and machine learning
Published: 2025-04-24
Journal: Scientific Reports
Application: WB
Impact Factor: 3.90
Species: Human,Rat
PMID: 40274967
Review
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Product Promise
We promise Anti-GLIPR2 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IP, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A10232-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026