Anti-GLS2 Antibody

Product Name

Anti-GLS2 Antibody

View all GLS2 Antibodies

SKU/Catalog Number

A05334

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GLS2 Antibody (Catalog # A05334). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

GLS2 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-GLS2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05334)

Host

Rabbit

Contents

GLS2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2. The immunogen is located within amino acids 300 - 350 of GLS2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Multiple isoforms of GLS2 are known to exist.

Reactive Species

A05334 is reactive to GLS2 in Human, Mouse, Rat

Applications

A05334 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

66.323kDa

Background of GLS2

Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of GLS2 in rat liver tissue lysate with GLS2 antibody at (A) 1 and (B) 2 μg/mL.

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Immunohistochemistry of GLS2 in rat liver tissue with GLS2 antibody at 5 μg/mL.

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Immunofluorescence of GLS2 in rat liver cells with GLS2 antibody at 20 5 μg/mL.

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Immunohistochemistry of GLS2 in mouse brain tissue with GLS2 Antibody at 5 μg/mL.

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Immunofluorescence of GLS2 in mouse brain tissue with GLS2 Antibody at 20 μg/mL.

Green: GLS2 antibody (A05334)
Red: Phylloidin staining
Blue: DAPI staining

Gene/Protein Information For GLS2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.5.1.2; GAbreast cell glutaminase; GLSglutaminase GA; glutaminase 2 (liver, mitochondrial); glutaminase I; glutaminase liver isoform, mitochondrial; hLGA; LGA; L-glutaminase; L-glutamine amidohydrolase; MGC71567; phosphate-activated glutaminase; phosphate-dependent glutaminase; truncated glutaminase 2 GLS2 GA, GLS, LGA, hLGA glutaminase 2 glutaminase liver isoform, mitochondrial|L-glutamine amidohydrolase|breast cell glutaminase|glutaminase 2 (liver, mitochondrial)|glutaminase I|phosphate-activated glutaminase|phosphate-dependent glutaminase

For more info on GLS2, check out the GLS2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GLS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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