Click image to see more details
Product Info Summary
| SKU: | PA2167 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GLUT12/SLC2A12 Antibody
SKU/Catalog Number
PA2167
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GLUT12/SLC2A12 Antibody catalog # PA2167. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GLUT12/SLC2A12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2167)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human GLUT12, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2167 is reactive to SLC2A12 in Human, Mouse, Rat
Applications
PA2167 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
55 kDa
Calculated molecular weight
66.966kDa
Background of GLUT12
Solute carrier family 2, facilitated glucose transporter member 12, also known as SLC2A12, is a protein that in humans is encoded by the SLC2A12 gene. SLC2A12 belongs to a family of transporters that catalyze the uptake of sugars through facilitated diffusion. This family of transporters shows conservation of 12 transmembrane helices as well as functionally significant amino acid residues. By genomic sequence analysis, this gene is mapped to chromosome 6q23.2. SLC2A12 was detected in a perinuclear location in MCF7 cells in the absence of insulin; however, SLC2A12 redistributed to the plasma membrane of MCF7 cells grown continuously in the presence of insulin. This gene acts as facilitative glucose transporter.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-GLUT12 antibody, PA2167, Western blotting
Lane 1: PC-12 Cell Lysate
Lane 2: A549 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For SLC2A12 (Source: Uniprot.org, NCBI)
Gene Name
SLC2A12
Full Name
Solute carrier family 2, facilitated glucose transporter member 12
Weight
66.966kDa
Superfamily
major facilitator superfamily
Alternative Names
GLUT-12; GLUT12GLUT8Glucose transporter type 12; solute carrier family 2 (facilitated glucose transporter), member 12; solute carrier family 2, facilitated glucose transporter member 12 SLC2A12 GLUT12, GLUT8 solute carrier family 2 member 12 solute carrier family 2, facilitated glucose transporter member 12|GLUT-12|glucose transporter type 12|solute carrier family 2 (facilitated glucose transporter), member 12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC2A12, check out the SLC2A12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC2A12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GLUT12/SLC2A12 Antibody (PA2167)
Hello CJ!
No publications found for PA2167
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Recommended Resources
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-GLUT12/SLC2A12 Antibody
Question
I was wanting to use your anti-GLUT12/SLC2A12 antibody for WB for mouse pigmented layer of retina on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse pigmented layer of retina identification?
D. Krishna
Verified customer
Asked: 2020-04-17
Answer
You can see on the product datasheet, PA2167 anti-GLUT12/SLC2A12 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse pigmented layer of retina in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-17
Question
We are currently using anti-GLUT12/SLC2A12 antibody PA2167 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
The anti-GLUT12/SLC2A12 antibody (PA2167) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-20
Question
I see that the anti-GLUT12/SLC2A12 antibody PA2167 works with WB, what is the protocol used to produce the result images on the product page?
A. Rodriguez
Verified customer
Asked: 2016-03-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-03-16
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pigmented layer of retina using anti-GLUT12/SLC2A12 antibody PA2167. Let me know if you need anything else.
O. Krishna
Verified customer
Asked: 2015-07-21
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-21
