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Product Info Summary
| SKU: | M02297-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-GLUT2/SLC2A2 Antibody (Monoclonal, 31S34)
SKU/Catalog Number
M02297-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-GLUT2/SLC2A2 Antibody (Monoclonal, 31S34) catalog # M02297-2. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GLUT2/SLC2A2 Antibody (Monoclonal, 31S34) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02297-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
31S34
Immunogen
Synthetic peptide within Human Glucose Transporter GLUT2 aa 37-82.
Reactive Species
M02297-2 is reactive to SLC2A2 in Human, Mouse, Rat
Calculated molecular weight
57.5 kDa
Background of SLC2A2
SLC2A2, also known as Glucose transporter 2 (GLUT2), is a transmembrane carrier protein that enables protein facilitatedglucose movement across cell membranes. This gene encodes an integral plasma membrane glycoprotein of the liver, islet beta cells, intestine, and kidney epithelium. The encoded protein mediates facilitated bidirectional glucose transport. Because of its low affinity for glucose, it has been suggested as a glucose sensor. Mutations in this gene are associated with susceptibility to diseases, including Fanconi-Bickel syndrome and noninsulin-dependent diabetes mellitus (NIDDM). Alternative splicing results in multiple transcript variants of this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02297-2 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Flow Cytometry (Fixed), 1:50-200
Validation Images & Assay Conditions
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Western blot analysis of GLUT2/SLC2A2 using anti-GLUT2/SLC2A2 antibody (M02297-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT2/SLC2A2 antigen affinity purified monoclonal antibody (M02297-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GLUT2/SLC2A2 at approximately 55 kDa. The expected band size for GLUT2/SLC2A2 is at 57 kDa.
Specific Publications For Anti-GLUT2/SLC2A2 Antibody (Monoclonal, 31S34) (M02297-2)
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