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- Table of Contents
GLS antibody (A01272-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Flow Cytometry analysis of 293T cells using anti-Glutaminase/GLS antibody (A01272-2).
Overlay histogram showing 293T cells stained with A01272-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glutaminase/GLS Antibody (A01272-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing 293T cells stained with A01272-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glutaminase/GLS Antibody (A01272-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Glutaminase/GLS Antibody Picoband®
GLS antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
GLS antibody was positively detected by WB in:
human SiHa whole cell, human A549 whole cell, human HEL whole cell, human U251 whole cell, human U2OS whole cell, human Hela whole cell, human K562 whole cell, human 293T whole cell, rat brain tissue, rat kidney tissue, rat heart tissue, mouse brain tissue, mouse kidney tissue, mouse heart tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GLS antibody was positively detected by IHC in:
human gastric cancer tissue, rat brain tissue, human liver cancer, mouse brain, mouse kidney,
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
GLS antibody was positively detected by ICC/IF in:
U20S cell
GLS antibody was positively detected by IF in:
human endometrial cancer, human liver cancer, mouse cerebellum, mouse kidney
GLS antibody was positively detected by FC in:
293T cell, SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat
Immunocytochemistry/Immunofluorescence
4μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Tested
Recommend
Monkey
Recommend
Rabbit
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Glutaminase/GLS Antibody Picoband®
View all GLS Antibodies
View all GLS Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Glutaminase/GLS recombinant protein (Position: K396-N654).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated Molecular Weight
73.5 kDa
Background of GLS
This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
Cite This Product
Anti-Glutaminase/GLS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01272-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Monkey
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Dog
99%
High Homology
Zebrafish
88%
Moderate Homology
Drosophila
57%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GLS antibody
Immunohistochemistry (IHC) protocol for GLS antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for GLS antibody
Immunofluorescence (IF) protocol for GLS antibody
Flow Cytometry (FC) protocol for GLS antibody
Publications
Application:
Species:
Phosphate binders suppress glutaminase activity for treatment of cirrhosis and hepatic encephalopathy murine models
Published: 2026-01-24
Journal: BIOCHEMICAL PHARMACOLOGY
Application: WB,IF
Impact Factor: 5.60
Species: Human,Rat
PMID: 41587593
Microbiome and spatially resolved metabolomics analysis reveal the anticancer role of gut Akkermansia muciniphila by crosstalk with intratumoral microbiota and reprogramming tumoral metabolism in mice
Published: 2023-02-05
Journal: Gut Microbes
Application: IHC
Impact Factor: 12.20
Species: Mouse
PMID: 36740846
Safflower Yellow Improves the Synaptic Structural Plasticity by Ameliorating the Disorder of Glutamate Circulation in Aβ1-42-induced AD Model Rats
Published: 2020-05-14
Journal: NEUROCHEMICAL RESEARCH
Application: WB
Impact Factor: 3.04
Species: Rat
PMID: 32410043
Rolling circle amplification integrated with suspension bead array for ultrasensitive multiplex immunodetection of tumor markers
Published: 2018-10-04
Journal: ANALYTICA CHIMICA ACTA
Application: FC
Impact Factor: 5.12
Species: Human
PMID: 30598160
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Product Promise
We promise Anti-Glutaminase/GLS Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01272-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026