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- Table of Contents
GLS Antibodies
AND GLS ELISA kits, GLS Proteins
Many biological assays use antibodies to detect GLS, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with GLS in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop GLS antibodies...
Designing a Western blot for GLS? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the GLS Western Blot GuideGLS Infographic by Boster Bio
All GLS Products
Product
Figures
Specificity
Applications
Price
Anti-Glutaminase/GLS Antibody Picoband®
4 Citations
Human, Monkey, Mouse, Rat
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A01272-2
100 μg/vial
Cat # A01272-2
100 μg/vial
Human, Mouse, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # M01272-3
100 μg/vial
Cat # M01272-3
100 μg/vial
Human, Mouse, Rat
IF, IHC, ICC, WB
$370
Cat # M01272-2
100 μg/vial
Cat # M01272-2
100 μg/vial
Human, Mouse, Rat
IF, IHC, ICC, WB
$370
Cat # M01272
100 μl
Cat # M01272
100 μl
Human, Mouse, Rat
ELISA, IP, IF, IHC, WB
$146
Cat # M01272-4
20μL, 40μL, 100μL, 200μL
Cat # M01272-4
20μL, 40μL, 100μL, 200μL
Anti-GLS2 Antibody Picoband®
1 Citations
Human
ELISA, Flow Cytometry, WB
$370
Cat # A05334-1
100 μg/vial
Cat # A05334-1
100 μg/vial
GLS Overview
Facts about Glutaminase kidney isoform, mitochondrial.
Glutaminase kidney isoform, mitochondrial (GLS)
Catalyzes the initial reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in keeping acid-base homeostasis.
Regulates the amount of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
Gene Information
| Human | |
|---|---|
| Gene Name: | GLS |
| Uniprot: | O94925 |
| Entrez: | 2744 |
Family
| Belongs to: |
|---|
| glutaminase family |
Alternative Names
AAD20; EC 3.5.1.2; GLS; GLS1DKFZp686O15119; glutaminase C; glutaminase kidney isoform, mitochondrial; Glutaminase; glutaminase, phosphate-activated; K-Glutaminase; KIAA0838FLJ10358; L-Glutamine Amidohydrolase
Molecular Weight
Mass (kDA):
73.461 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 2q32.2 |
| Sequence: | 2; NC_000002.12 (190880821..190965552) |
Tissue Specificity
Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.
Subcellular Localizations
[Isoform 1]: Mitochondrion. Cytoplasm, cytosol. The 74-kDa cytosolic precursor is translocated into the mitochondria and processed via a 72-kDa intermediate to yield the mature 68- and 65-kDa subunits.; [Isoform 3]: Mitochondrion.; [Glutaminase kidney isoform, mitochondrial 68 kDa chain]: Mitochondrion matrix. Produced by the proteolytic processing of the 74-kDa cytosolic precursor.; [Glutaminase kidney isoform, mitochondrial 65 kDa chain]: Mitochondrion matrix. Produced by the proteolytic processing of the 74-kDa cytosolic precursor.
Diseases
- Ventricular Dysfunction, Left
- Infectious Bursal Disease
- Stenosis
- Neoplasms
- Heart Failure
- Aortic Valve Stenosis
- Rheumatoid Arthritis
- Malignant Neoplasms
- Arthritis
- Poultry Diseases
- Cardiomyopathies
- Hypertrophy
Pathways
- Cell Growth
- Localization
- Pathogenesis
- Glycolysis
- Fermentation
- Mating
- Cell Proliferation
- Transport
- Virulence
- Secretion
- Cell Adhesion
- Transposition
- Golgi Localization
PTMs
- Phosphorylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/GLS
bosterbio.com/bosterbio-gene-info-cards/GLS
References
PMID: 11015561 by Elgadi K.M., et al. Cloning and analysis of unique human glutaminase isoforms generated by tissue-specific alternative splicing.
PMID: 10719215 by Holcomb T., et al. Isolation, characterization and expression of a human brain mitochondrial glutaminase cDNA.