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Product Info Summary
| SKU: | M01272 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Glutaminase GLS Rabbit Monoclonal Antibody
SKU/Catalog Number
M01272
BM4558 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Glutaminase GLS Rabbit Monoclonal Antibody catalog # M01272. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glutaminase GLS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01272)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FIA-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Glutaminase
Reactive Species
M01272 is reactive to GLS in Human, Mouse, Rat
Observed Molecular Weight
65, 73 kDa
Calculated molecular weight
73.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01272 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: 293T cell
IHC: human bladder cancer tissue, human liver cancer tissue, human colon cancer tissue, human thyroid cancer tissue, human renal cancer tissue, human cervical cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Glutaminase expression in 293T cell lysate.
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IHC analysis of GLS using anti-GLS antibody (M01272).
GLS was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLS Antibody (M01272) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of GLS using anti-GLS antibody (M01272).
GLS was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLS Antibody (M01272) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of GLS using anti-GLS antibody (M01272).
GLS was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLS Antibody (M01272) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of GLS using anti-GLS antibody (M01272).
GLS was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLS Antibody (M01272) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of GLS using anti-GLS antibody (M01272).
GLS was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLS Antibody (M01272) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of GLS using anti-GLS antibody (M01272).
GLS was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLS Antibody (M01272) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Glutaminase GLS Rabbit Monoclonal Antibody (M01272)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Glutaminase GLS Rabbit Monoclonal Antibody
Question
Would anti-Glutaminase Rabbit Monoclonal antibody M01272 work for IHC with brain?
V. Miller
Verified customer
Asked: 2019-04-19
Answer
According to the expression profile of brain, GLS is highly expressed in brain. So, it is likely that anti-Glutaminase Rabbit Monoclonal antibody M01272 will work for IHC with brain.
Boster Scientific Support
Answered: 2019-04-19
Question
Do you have a BSA free version of anti-Glutaminase Rabbit Monoclonal antibody M01272 available?
Verified Customer
Verified customer
Asked: 2019-01-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Glutaminase Rabbit Monoclonal antibody M01272 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-30
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Glutaminase Rabbit Monoclonal antibody M01272. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-10
Question
Does M01272 anti-Glutaminase Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-26
Answer
You can see on the product datasheet, M01272 anti-Glutaminase Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-26
Question
We are currently using anti-Glutaminase Rabbit Monoclonal antibody M01272 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
The anti-Glutaminase Rabbit Monoclonal antibody (M01272) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-05
Question
Is this M01272 anti-Glutaminase Rabbit Monoclonal antibody reactive to the isotypes of GLS?
S. Li
Verified customer
Asked: 2014-01-13
Answer
The immunogen of M01272 anti-Glutaminase Rabbit Monoclonal antibody is A synthesized peptide derived from human Glutaminase. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-01-13
