Anti-Glycine decarboxylase/GLDC Antibody Picoband™ (monoclonal, 3D3D3)

Product Name

Anti-Glycine decarboxylase/GLDC Antibody Picoband™ (monoclonal, 3D3D3)

View all GLDC Antibodies

SKU/Catalog Number

M04777

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Glycine decarboxylase/GLDC Antibody Picoband™ (monoclonal, 3D3D3) catalog # M04777. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Glycine decarboxylase/GLDC Antibody Picoband™ (monoclonal, 3D3D3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04777)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

3D3D3

Isotype

Mouse IgG1

Immunogen

E.coli-derived human Glycine decarboxylase/GLDC recombinant protein (Position: K574-S1020).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M04777 is reactive to GLDC in Human, Mouse, Rat

Applications

M04777 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

113 kDa

Calculated molecular weight

112.73kDa

Background of GLDC

Glycine decarboxylase also known as glycine cleavage system P protein or glycine dehydrogenase is an enzyme that in humans is encoded by the GLDC gene. Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of GLDC using anti-GLDC antibody (M04777).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCP tissue lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GLDC antigen affinity purified monoclonal antibody (Catalog # M04777) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GLDC at approximately 113 kDa. The expected band size for GLDC is at 113 kDa.

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Figure 2. IHC analysis of GLDC using anti-GLDC antibody (M04777).
GLDC was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-GLDC Antibody (M04777) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

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Figure 3. IHC analysis of GLDC using anti-GLDC antibody (M04777).
GLDC was detected in a paraffin-embedded section of human thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-GLDC Antibody (M04777) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

Gene/Protein Information For GLDC (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.4.4.2; GCE; GCSPMGC138198; Glycine cleavage system P protein; glycine cleavage system protein P; glycine decarboxylase P-protein; Glycine decarboxylase; glycine dehydrogenase (decarboxylating); glycine dehydrogenase (decarboxylating; glycine decarboxylase, glycine cleavagesystem protein P); glycine dehydrogenase [decarboxylating], mitochondrial; HYGN1; MGC138200; NKH GLDC GCE, GCSP, HYGN1 glycine decarboxylase glycine dehydrogenase (decarboxylating), mitochondrial|glycine cleavage system protein P|glycine decarboxylase P-protein|glycine dehydrogenase (aminomethyl-transferring)|glycine dehydrogenase [decarboxylating], mitochondrial|nonketotic hyperglycinemia

For more info on GLDC, check out the GLDC Infographic

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In this infographic, you will see the following information for GLDC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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