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Product Info Summary
| SKU: | M14911 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-GMCL1 Antibody (Center)
SKU/Catalog Number
M14911
Size
50 µl
Description
Boster Bio Anti-GMCL1 Antibody (Center) (Catalog # M14911). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GMCL1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14911)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This GMCL1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 281-315 amino acids from the Central region of human GMCL1.
Reactive Species
M14911 is reactive to GMCL1 in Human, Mouse
Calculated molecular weight
58.7 kDa
Background of GMCL1
Possible function in spermatogenesis. Enhances the degradation of MDM2 and increases the amount of p53 probably by modulating the nucleocytoplasmic transport (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M14911 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-GMCL1 Antibody (Center) at 1:2000 dilution
Lane 1: 293T/17 whole cell lysate
Lane 2: HL-60 whole cell lysate
Lane 3: mouse testis lysate
Lane 4: MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 59 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M14911 staining GMCL1 in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-GMCL1 Antibody (Center) (M14911)
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