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Product Info Summary
| SKU: | M06975 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-GNG2 Antibody (N-Term)
SKU/Catalog Number
M06975
Size
50 µl
Description
Boster Bio Anti-GNG2 Antibody (N-Term) (Catalog # M06975). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GNG2 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06975)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This GNG2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 19-52 amino acids from of human GNG2.
Reactive Species
M06975 is reactive to GNG2 in Human, Mouse
Calculated molecular weight
7.9 kDa
Background of GNG2
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06975 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:8000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-GNG2 Antibody (N-Term) at 1:8000 dilution
Lane 1: human brain lysate
Lane 2: mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 8 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M06975 staining GNG2 in human brain tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-GNG2 Antibody (N-Term) (M06975)
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