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Product Info Summary
| SKU: | M03562 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-GNMT Antibody (C-term)
SKU/Catalog Number
M03562
Size
50 µl
Description
Boster Bio Anti-GNMT Antibody (C-term) (Catalog # M03562). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GNMT Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03562)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1041CT18.2.3
Isotype
IgG2a,k
Immunogen
This GNMT antibody is generated from a mouse immunized with a recombinant protein between 1-295 amino acids from the human GNMT.
Reactive Species
M03562 is reactive to GNMT in Human
Calculated molecular weight
32.7 kDa
Background of GNMT
Catalyzes the methylation of glycine by using S- adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03562 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
FC: 1:25
Validation Images & Assay Conditions
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Anti-GNMT Antibody (C-term) at 1:4000 dilution + Human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 33 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing U-2 OS cells stained with M03562(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M03562, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Specific Publications For Anti-GNMT Antibody (C-term) (M03562)
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