Anti-GOLGIN97 GOLGA1 Antibody

Product Name

Anti-GOLGIN97 GOLGA1 Antibody

View all GOLGA1 Antibodies

SKU/Catalog Number

A13524

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GOLGIN97 GOLGA1 Antibody (Catalog # A13524). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

GOLGIN97 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-GOLGIN97 GOLGA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13524)

Host

Rabbit

Contents

GOLGIN97 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

GOLGIN97 antibody was raised against a 17 amino acid peptide near the amino terminus of human GOLGIN97. The immunogen is located within amino acids 30 - 80 of GOLGIN97.

Cross-reactivity

GOLGIN97 antibody is human, mouse and rat reactive. At least two isoforms of GOLGIN97 are known to exist; this antibody will detect only the longer isoform. GOLGIN97 antibody is predicted to not cross-react with other GOLGIN family proteins.

Reactive Species

A13524 is reactive to GOLGA1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

88.2 kDa

Background of GOLGA1

GOLGIN97 was initially isolated as a Golgi complex autoantigen associated with Sjogren's syndrome, a chronic autoimmune disease in which the body's white blood cells destroy the exocrine glands, specifically the salivary and lacrimal glands (1,2). GOLGIN97 plays a role as a tethering molecule in endosome to the trans-Golgi network (TGN) retrograde traffic (3). FIP1/RCP binding to GOLGIN97 is required for tethering and fusion of recycling endosome retrograde transport vesicles to the TGN (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A13524 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

GOLGIN97 antibody can be used for detection of GOLGIN97 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information GOLGA1

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