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Product Info Summary
| SKU: | A30705 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GORASP2/Grasp55 Antibody
SKU/Catalog Number
A30705
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GORASP2/Grasp55 Antibody (Catalog# A30705). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GORASP2/Grasp55 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30705)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GORASP2. Immunogen sequence location: 181-230
Reactive Species
A30705 is reactive to GORASP2 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
47.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30705 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of various cells using GRASP55 Polyclonal Antibody
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Western blot analysis of lysates from HeLa, HepG2, Jurkat, and LOVO cells, using GORASP2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GORASP2/Grasp55 Antibody (A30705)
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Customer Q&As
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1 Customer Q&As for Anti-GORASP2/Grasp55 Antibody
Question
We are currently using anti-GORASP2/Grasp55 antibody A30705 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
The anti-GORASP2/Grasp55 antibody (A30705) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-03
