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- Table of Contents
GPAA1 antibody (A09298-1) performs well in ELISA, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of GPAA1 using anti-GPAA1 antibody (A09298-1).
GPAA1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPAA1 Antibody (A09298-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
GPAA1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPAA1 Antibody (A09298-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-GPAA1 Antibody Picoband®
GPAA1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IP, IF, IHC, ICC, WB |
Tested Applications
GPAA1 antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell, human HUH-7 whole cell, human MCF-7 whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell, HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GPAA1 antibody was positively detected by IHC in:
human breast cancer tissue, human colon cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
GPAA1 antibody was positively detected by ICC/IF in:
MCF-7 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
ELISA
Human
Tested
Tested
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-GPAA1 Antibody Picoband®
View all GPAA1 Antibodies
View all GPAA1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human GPAA1 recombinant protein (Position: L101-Q355).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
67.6 kDa
Background of GPAA1
Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains.
Cite This Product
Anti-GPAA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09298-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Rat
96%
High Homology
Rabbit
96%
High Homology
Cow
96%
High Homology
Sheep
96%
High Homology
Dog
96%
High Homology
Mouse
95%
High Homology
Zebrafish
69%
Low homology
Drosophila
43%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GPAA1 antibody
Immunohistochemistry (IHC) protocol for GPAA1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for GPAA1 antibody
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Product Promise
We promise Anti-GPAA1 Antibody Picoband® performs excellently in relevant applications for ELISA, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A09298-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026