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Product Info Summary
| SKU: | A11699 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-GPR158 Antibody
SKU/Catalog Number
A11699
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR158 Antibody catalog # A11699. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR158 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11699)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR158. AA range:1-50
Reactive Species
A11699 is reactive to GPR158 in Human, Mouse
Calculated molecular weight
135.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11699 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Immunofluorescence analysis of HUVEC cells, using GPR158 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of COLO 293T HELA cells using GPR158 Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from K562 cells, using GPR158 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GPR158 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR158 Antibody (A11699)
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Customer Reviews
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7 Customer Q&As for Anti-GPR158 Antibody
Question
My question regards to test anti-GPR158 antibody A11699 on mouse caudate nucleus for research purposes, then I may be interested in using anti-GPR158 antibody A11699 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Zhao
Verified customer
Asked: 2020-01-13
Answer
The products we sell, including anti-GPR158 antibody A11699, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-13
Question
Will A11699 anti-GPR158 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
As indicated on the product datasheet, A11699 anti-GPR158 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-17
Question
I was wanting to use your anti-GPR158 antibody for WB for mouse caudate nucleus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse caudate nucleus identification?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
As indicated on the product datasheet, A11699 anti-GPR158 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse caudate nucleus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-26
Question
Is a blocking peptide available for product anti-GPR158 antibody (A11699)?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
We do provide the blocking peptide for product anti-GPR158 antibody (A11699). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-11-13
Question
I see that the anti-GPR158 antibody A11699 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-01
Question
Is this A11699 anti-GPR158 antibody reactive to the isotypes of GPR158?
P. Rodriguez
Verified customer
Asked: 2016-09-28
Answer
The immunogen of A11699 anti-GPR158 antibody is Synthesized peptide derived from the N-terminal region of human GPR158. at AA rangle: 10-90. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-09-28
Question
Is there a BSA free version of anti-GPR158 antibody A11699 available?
G. Moore
Verified customer
Asked: 2013-01-31
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-GPR158 antibody A11699 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-01-31
