Anti-GPR171 Antibody

GPR171 antibody

Boster Bio Anti-GPR171 Antibody catalog # A14498. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A14498
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, ICC, WB

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Product Name

Anti-GPR171 Antibody

View all GPR171 Antibodies

SKU/Catalog Number

A14498

Size

100μl

Form

Liquid

Description

Boster Bio Anti-GPR171 Antibody catalog # A14498. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GPR171 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14498)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

The antiserum was produced against synthesized peptide derived from human GPR171 at amino acid range 181-230

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14498 is reactive to GPR171 in Human, Mouse, Rat

Applications

A14498 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

36.754kDa

Background of GPR171

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H4 pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000

Validation Images & Assay Conditions

Gene/Protein Information For GPR171 (Source: Uniprot.org, NCBI)

Gene Name

GPR171

Full Name

Probable G-protein coupled receptor 171

Weight

36.754kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

F730001G15Rik; G protein-coupled receptor 171; GPR171; G-protein coupled receptor H963; H963; H963platelet activating receptor homolog; probable G-protein coupled receptor 171 GPR171 H963 G protein-coupled receptor 171 probable G-protein coupled receptor 171|F730001G15Rik|G-protein coupled receptor H963|platelet activating receptor homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GPR171, check out the GPR171 Infographic

GPR171 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GPR171: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-GPR171 Antibody

Question

My question regarding product A14498, anti-GPR171 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14498 anti-GPR171 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-10

Question

Is there a BSA free version of anti-GPR171 antibody A14498 available?

Verified Customer

Verified customer

Asked: 2019-10-18

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-GPR171 antibody A14498 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-18

Question

Is a blocking peptide available for product anti-GPR171 antibody (A14498)?

Verified Customer

Verified customer

Asked: 2018-08-13

Answer

We do provide the blocking peptide for product anti-GPR171 antibody (A14498). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-08-13

Question

Will A14498 anti-GPR171 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Walker

Verified customer

Asked: 2015-08-26

Answer

As indicated on the product datasheet, A14498 anti-GPR171 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-08-26

Order DetailsPrice
A14498

100uL

$399

Free Secondary Antibody

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A14498
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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