Product Info Summary
| SKU: | A14498 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-GPR171 Antibody
SKU/Catalog Number
A14498
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR171 Antibody catalog # A14498. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR171 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14498)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR171. AA range:181-230
Reactive Species
A14498 is reactive to GPR171 in Human, Mouse, Rat
Calculated molecular weight
36.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14498 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of various cells using GPR171 Polyclonal Antibody
Specific Publications For Anti-GPR171 Antibody (A14498)
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4 Customer Q&As for Anti-GPR171 Antibody
Question
My question regarding product A14498, anti-GPR171 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14498 anti-GPR171 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-10
Question
Is there a BSA free version of anti-GPR171 antibody A14498 available?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-GPR171 antibody A14498 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-18
Question
Is a blocking peptide available for product anti-GPR171 antibody (A14498)?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
We do provide the blocking peptide for product anti-GPR171 antibody (A14498). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-08-13
Question
Will A14498 anti-GPR171 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Walker
Verified customer
Asked: 2015-08-26
Answer
As indicated on the product datasheet, A14498 anti-GPR171 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-08-26

