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- Table of Contents
ITGB5 antibody (M04201) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of A549 cells using anti-Integrin beta 5/ITGB5 antibody (M04201).
Overlay histogram showing A549 cells stained with M04201 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti- Integrin beta 5/ITGB5 Antibody (M04201, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A549 cells stained with M04201 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti- Integrin beta 5/ITGB5 Antibody (M04201, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Integrin beta 5/ITGB5 Antibody Picoband® (monoclonal, 9E2)
ITGB5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | Flow Cytometry, IHC, WB |
Tested Applications
ITGB5 antibody was positively detected by WB in:
human A549 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ITGB5 antibody was positively detected by IHC in:
human lung cancer tissue, human ovarian serous adenocarcinoma tissue, human rectal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ITGB5 antibody was positively detected by FC in:
A549 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Integrin beta 5/ITGB5 Antibody Picoband® (monoclonal, 9E2)
View all ITGB5 Antibodies
View all ITGB5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Monoclonal
Clone Number
9E2
Isotype
Mouse IgG1
Immunogen
E.coli-derived human Integrin beta 5/ITGB5 recombinant protein (Position: S32-D689).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated Molecular Weight
88.1 kDa
Background of ITGB5
This gene encodes a beta subunit of integrin, which can combine with different alpha chains to form a variety of integrin heterodimers. Integrins are integral cell-surface receptors that participate in cell adhesion as well as cell-surface mediated signaling. The alphav beta5 integrin is involved in adhesion to vitronectin.
Cite This Product
Anti-Integrin beta 5/ITGB5 Antibody Picoband® (monoclonal, 9E2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04201)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Rabbit
93%
High Homology
Cow
92%
High Homology
Mouse
92%
High Homology
Rat
92%
High Homology
Sheep
91%
High Homology
Dog
90%
High Homology
Zebrafish
74%
Moderate Homology
Drosophila
41%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ITGB5 antibody
Immunohistochemistry (IHC) protocol for ITGB5 antibody
Flow Cytometry (FC) protocol for ITGB5 antibody
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Product Promise
We promise Anti-Integrin beta 5/ITGB5 Antibody Picoband® (monoclonal, 9E2) performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M04201
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026