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- Table of Contents
KISS1R antibody (A01364-2) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of KISS1R using anti-KISS1R antibody (A01364-2).
KISS1R was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KISS1R Antibody (A01364-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
KISS1R was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KISS1R Antibody (A01364-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-GPR54/KISS1R Antibody Picoband®
KISS1R antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC, ICC, WB |
Tested Applications
KISS1R antibody was positively detected by WB in:
human MCF-7 whole cell, human Hela whole cell, human HepG2 whole cell, rat kindey tissue, mouse pancreas tissue, mouse kindey tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
KISS1R antibody was positively detected by IHC in:
human gall bladder adenosquamous carcinoma tissue, human hyroid papillary carcinoma tissue, human liver cancer tissue, mouse colon tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
KISS1R antibody was positively detected by ICC/IF in:
CACO-2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
1-2 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-GPR54/KISS1R Antibody Picoband®
View all KISS1R Antibodies
View all KISS1R Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GPR54/KISS1R recombinant protein (Position: N60-A373).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
80 kDa
Calculated Molecular Weight
42.6 kDa
Background of KISS1R
The KiSS1-derived peptide receptor (also known as GPR54 or the Kisspeptin receptor) is a G protein-coupled receptor which binds the peptide hormone kisspeptin (metastin). Kisspeptin is involved in the regulation of endocrine function and the onset of puberty, with activation of the kisspeptin receptor triggering release of gonadotropin-releasing hormone (GnRH), and release of kisspeptin itself being inhibited by oestradiol but enhanced by GnRH. Reductions in kisspeptin levels with age may conversely be one of the reasons behind age-related declines in levels of other endocrine hormones such as luteinizing hormone.
Cite This Product
Anti-GPR54/KISS1R Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01364-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Mouse
88%
Moderate Homology
Rat
88%
Moderate Homology
Dog
88%
Moderate Homology
Cow
88%
Moderate Homology
Sheep
87%
Moderate Homology
Zebrafish
52%
Low homology
Drosophila
33%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for KISS1R antibody
Immunohistochemistry (IHC) protocol for KISS1R antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for KISS1R antibody
Review
Have you used Anti-GPR54/KISS1R Antibody Picoband®?
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1 Review For Anti-GPR54/KISS1R Antibody Picoband®
Anti-GPR54 Antibody (A01364-2) produced a clear and specific band at the expected molecular weight with minimal background, demonstrating excellent performance for detecting GPR54 in rat hypothalamus by Western blot.
| SKU | A01364-2 |
|---|---|
| Application | Western Blot |
| Sample | rat hypothalamus tissue |
| Sample Processing Description | Total proteins were extracted from normally cultured HepG2 cells. |
| Other Reagents | ① Hypothalamus of non-estrous rats ② Hypothalamus of estrous rats. Total protein extraction. |
| Primary Antibody | GPR54/KISS1R Antibody Picoband® |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | GPR45 protein is the "master switch" of the mammalian reproductive neuroendocrine system and the "ignition key" for puberty. Theoretically, its content in mammals is significantly higher during estrus than during non-estrus. Experimental results have also confirmed this. |
Yanlin Zhang, Shanxi Medical University
Verified customer
Submitted 2026-07-08
Product Promise
We promise Anti-GPR54/KISS1R Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01364-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026