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Product Info Summary
| SKU: | A13384 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-GPR75 Antibody
SKU/Catalog Number
A13384
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR75 Antibody catalog # A13384. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR75 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13384)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR75. AA range:381-430
Reactive Species
A13384 is reactive to GPR75 in Human, Mouse
Calculated molecular weight
59.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13384 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Immunofluorescence analysis of LOVO cells, using GPR75 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of HT-29 cells using GPR75 Polyclonal Antibody
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Western blot analysis of lysates from HT-29 and K562 cells, using GPR75 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR75 Antibody (A13384)
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Customer Q&As
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4 Customer Q&As for Anti-GPR75 Antibody
Question
you antibody to test anti-GPR75 antibody A13384 on mouse corpus callosum for research purposes, then I may be interested in using anti-GPR75 antibody A13384 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Wu
Verified customer
Asked: 2020-02-06
Answer
The products we sell, including anti-GPR75 antibody A13384, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-06
Question
I was wanting to use your anti-GPR75 antibody for WB for mouse corpus callosum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse corpus callosum identification?
Verified Customer
Verified customer
Asked: 2018-09-21
Answer
You can see on the product datasheet, A13384 anti-GPR75 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse corpus callosum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-21
Question
We are currently using anti-GPR75 antibody A13384 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-16
Answer
The anti-GPR75 antibody (A13384) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-16
Question
I see that the anti-GPR75 antibody A13384 works with WB, what is the protocol used to produce the result images on the product page?
K. Collins
Verified customer
Asked: 2015-07-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-07-07
