Anti-GPX3 Antibody (Center)

Product Name

Anti-GPX3 Antibody (Center)

View all Glutathione Peroxidase 3/GPX3 Antibodies

SKU/Catalog Number

A02637

Size

80 µl

Description

Boster Bio Anti-GPX3 Antibody (Center) (Catalog # A02637). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-GPX3 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02637)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This GPX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-123 amino acids from the Central region of human GPX3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02637 is reactive to GPX3 in Human, Mouse

Applications

A02637 is guaranteed for IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

25.552kDa

Background of Glutathione Peroxidase 3/GPX3

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC-P: 1:50-1:100

Validation Images & Assay Conditions

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All lanes : Anti-GPX3 Antibody (Center) at 1:1000 dilution
Lane 1: M. plasma lysate

Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Observed band size : 22kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Western blot analysis of lysates from human kidney, plasma, mouse kidney, plasma and rat kidney tissue lysate (from left to right), using GPX3 Antibody (Center) (Cat. #A02637). A02637 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

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GPX3 Antibody (Center) (Cat. #A02637) western blot analysis in mouse kidney tissue lysates (35ug/lane). This demonstrates the GPX3 antibody detected the GPX3 protein (arrow).

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GPX3 Antibody (Center) (Cat. #A02637)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of GPX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Gene/Protein Information For GPX3 (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.11.1; EC 1.11.1.9; Extracellular glutathione peroxidase; glutathione peroxidase 3 (plasma); Glutathione Peroxidase 3; GPX3; GPx-3; GPXP; GPX-P; GSHPx-3; GSHPx-P; Plasma glutathione peroxidase GPX3 GPx-P, GSHPx-3, GSHPx-P glutathione peroxidase 3 glutathione peroxidase 3|GPx-3|extracellular glutathione peroxidase|glutathione peroxidase 3 (plasma)|plasma glutathione peroxidase|selenoprotein GPX3

For more info on GPX3, check out the GPX3 Infographic

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In this infographic, you will see the following information for GPX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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