Anti-Grancalcin/GCA Antibody Picoband™

Grancalcin antibody

Boster Bio Anti-Grancalcin/GCA Antibody Picoband™ catalog # A01237. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01237
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Grancalcin/GCA Antibody Picoband™

View all Grancalcin Antibodies

SKU/Catalog Number

A01237

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Grancalcin/GCA Antibody Picoband™ catalog # A01237. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Grancalcin/GCA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01237)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Grancalcin/GCA recombinant protein (Position: A42-I217).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01237 is reactive to GCA in Human, Mouse, Rat

Applications

A01237 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

24.01kDa

Background of Grancalcin

Grancalcin is a protein that in humans is encoded by the GCA gene. This gene is mapped to 2q24.2. This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml
Immunocytochemistry/Immunofluorescence, 5 μg/ml
Flow Cytometry(Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For GCA (Source: Uniprot.org, NCBI)

Gene Name

GCA

Full Name

Grancalcin

Weight

24.01kDa

Alternative Names

EF-hand calcium-binding protein; grancalcin, EF-hand calcium binding protein; grancalcin, penta-EF-hand protein GCA GCL grancalcin grancalcin|grancalcin, EF-hand calcium-binding protein|grancalcin, penta-EF-hand protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GCA, check out the GCA Infographic

GCA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Grancalcin/GCA Antibody Picoband™

Question

Is this A01237 anti-Grancalcin/GCA antibody reactive to the isotypes of GCA?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

The immunogen of A01237 anti-Grancalcin/GCA antibody is E. coli-derived human Grancalcin/GCA recombinant protein (Position: A42-I217). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-10

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain cortex using anti-Grancalcin/GCA antibody A01237. Let me know if you need anything else.

Z. Singh

Verified customer

Asked: 2019-11-05

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-05

Question

We are currently using anti-Grancalcin/GCA antibody A01237 for rat tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

G. Miller

Verified customer

Asked: 2017-01-16

Answer

The anti-Grancalcin/GCA antibody (A01237) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-01-16

Order DetailsPrice
A01237

100μg

$370
A01237-10ug

10μg sample (liquid)

$99
A01237-Biotin

100 μg Biotin conjugated

$570
A01237-Cy3

100 μg Cy3 conjugated

$570
A01237-Dylight488

100 μg Dylight488 conjugated

$570
A01237-Dylight550

100 μg Dylight550 conjugated

$570
A01237-Dylight594

100 μg Dylight594 conjugated

$570
A01237-FITC

100 μg FITC conjugated

$570
A01237-HRP

100 μg HRP conjugated

$570
A01237-APC

100 μg APC conjugated

$670
A01237-PE

100 μg PE conjugated

$670
A01237-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A01237
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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