Product Info Summary
SKU: | PA1738 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Granzyme B/GZMB Antibody
View all Granzyme B Antibodies
SKU/Catalog Number
PA1738
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Granzyme B/GZMB Antibody catalog # PA1738. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Granzyme B/GZMB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1738)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Granzyme B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1738 is reactive to GZMB in Human
Applications
PA1738 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
55 kDa
Calculated molecular weight
27.716kDa
Background of Granzyme B
Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are though to protect their host by inducing apoptosis of cells that bear on their surface "nonself" antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-Granzyme B antibody, PA1738, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: HEPA Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For GZMB (Source: Uniprot.org, NCBI)
Gene Name
GZMB
Full Name
Granzyme B
Weight
27.716kDa
Superfamily
peptidase S1 family
Alternative Names
C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GRB; GrzB; GZMB; HLP; SECT GZMB C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT granzyme B granzyme B|T-cell serine protease 1-3E|cathepsin G-like 1|cytotoxic T-lymphocyte proteinase 2|cytotoxic serine protease B|fragmentin 2|granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)|human lymphocyte protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GZMB, check out the GZMB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GZMB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Granzyme B/GZMB Antibody (PA1738)
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No publications found for PA1738
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Granzyme B/GZMB Antibody
Question
See attached the WB image, lot number and protocol we used for leukocyte using anti-Granzyme B/GZMB antibody PA1738. Please let me know if you require anything else.
A. Krishna
Verified customer
Asked: 2020-04-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-06
Question
Is there a BSA free version of anti-Granzyme B/GZMB antibody PA1738 available?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Granzyme B/GZMB antibody PA1738 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-09
Question
I see that the anti-Granzyme B/GZMB antibody PA1738 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-24
Question
Would PA1738 anti-Granzyme B/GZMB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
You can see on the product datasheet, PA1738 anti-Granzyme B/GZMB antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-30
Question
Would anti-Granzyme B/GZMB antibody PA1738 work for WB with leukocyte?
Verified Customer
Verified customer
Asked: 2018-12-31
Answer
According to the expression profile of leukocyte, GZMB is highly expressed in leukocyte. So, it is likely that anti-Granzyme B/GZMB antibody PA1738 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2018-12-31
Question
We are currently using anti-Granzyme B/GZMB antibody PA1738 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
B. Singh
Verified customer
Asked: 2017-07-06
Answer
The anti-Granzyme B/GZMB antibody (PA1738) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-06