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Product Info Summary
| SKU: | M00353-4 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Granzyme B/GZMB Antibody (Monoclonal, 34G74)
SKU/Catalog Number
M00353-4
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Granzyme B/GZMB Antibody (Monoclonal, 34G74) catalog # M00353-4. Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Granzyme B/GZMB Antibody (Monoclonal, 34G74) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00353-4)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
34G74
Immunogen
Recombinant protein within human Granzyme B aa 3-245.
Reactive Species
M00353-4 is reactive to GZMB in Human, Mouse
Observed Molecular Weight
30 kDa
Calculated molecular weight
27.7 kDa
Background of GZMB
Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00353-4 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Positive Control
WB: mouse small intestine tissue lysates
IHC: human spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of Granzyme B/GZMB using anti-Granzyme B/GZMB antibody (M00353-4).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse small intestine tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Granzyme B/GZMB antigen affinity purified monoclonal antibody (M00353-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Granzyme B/GZMB at approximately 30 kDa. The expected band size for Granzyme B/GZMB is at 28 kDa.
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IHC analysis of Granzyme B/GZMB using anti-Granzyme B/GZMB antibody (M00353-4).
Granzyme B/GZMB was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Granzyme B/GZMB Antibody (M00353-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Granzyme B/GZMB Antibody (Monoclonal, 34G74) (M00353-4)
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