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Product Info Summary
| SKU: | A01663 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-GRB10 Antibody
SKU/Catalog Number
A01663
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GRB10 Antibody (Catalog # A01663). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
GRB10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-GRB10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01663)
Host
Rabbit
Contents
GRB10 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
GRB10 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GRB10. The immunogen is located within amino acids 440 - 490 of GRB10.
Cross-reactivity
At least three isoforms of GRB10 are known to exist; this antibody will detect all three isoforms.
Reactive Species
A01663 is reactive to GRB10 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
67.2 kDa
Background of GRB10
GRB10 belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules and function in diverse cellular processes. GRB10 is a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of GRB10 inhibits tyrosine kinase activity and results in growth suppression. GRB10 can also interact with the L isoform of the proapoptotic protein Bim. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01663 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
GRB10 antibody can be used for detection of GRB10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of GRB10 in SK-N-SH cell lysate with GRB10 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of GRB10 in rat brain cells with GRB10 antibody at 20 μg/mL.
Specific Publications For Anti-GRB10 Antibody (A01663)
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