Anti-GRB2-related adapter protein Grap Antibody

Product Name

Anti-GRB2-related adapter protein Grap Antibody

View all GRAP Antibodies

SKU/Catalog Number

A09913

Size

100ul

Form

Liquid

Description

Boster Bio Anti-GRB2-related adapter protein Grap Antibody catalog # A09913. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GRB2-related adapter protein Grap Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09913)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Grap

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A09913 is reactive to GRAP in Human, Mouse, Rat

Applications

A09913 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

25.337kDa

Background of GRAP

Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin.

Ota T., Nat. Genet. 36:40-45(2004).
Gregory S.G., Nature 441:315-321(2006).
Mural R.J., Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blotting validation for Anti-GRB2-related adapter protein Grap Antibody A09913

Western Blot (WB) analysis of 293 cells using Grap polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For GRAP (Source: Uniprot.org, NCBI)

Alternative Names

GRB2-related adapter protein; GRB2-related adaptor protein; growth factor receptor-bound protein 2-related adaptor protein; MGC64880 GRAP DFNB114 GRB2 related adaptor protein GRB2-related adapter protein|growth factor receptor-bound protein 2-related adaptor protein

For more info on GRAP, check out the GRAP Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GRAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the GRAP Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis