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Product Info Summary
| SKU: | A00351 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-GRB2 Antibody (Y209)
SKU/Catalog Number
A00351
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-GRB2 Antibody (Y209) (Catalog # A00351). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GRB2 Antibody (Y209) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00351)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This GRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from human GRB2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00351 is reactive to GRB2 in Human
Reconstitution
Calculated molecular weight
25206 Da
Background of GRB2
GRB2 binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00351 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysate from Ramos cell line, using GRB2 Antibody (pY209). A00351 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GRB2 Antibody (Y209), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Protein Target Info & Infographic
Gene/Protein Information For GRB2 (Source: Uniprot.org, NCBI)
Gene Name
GRB2
Full Name
Growth factor receptor-bound protein 2
Weight
25206 Da
Superfamily
GRB2/sem-5/DRK family
Alternative Names
Growth factor receptor-bound protein 2, Adapter protein GRB2, Protein Ash, SH2/SH3 adapter GRB2, GRB2, ASH GRB2 ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2 growth factor receptor bound protein 2 growth factor receptor-bound protein 2|HT027|SH2/SH3 adapter GRB2|abundant SRC homology|epidermal growth factor receptor-binding protein GRB2|epididymis secretory sperm binding protein|growth factor receptor-bound protein 3|protein Ash
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRB2, check out the GRB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GRB2 Antibody (Y209) (A00351)
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