Anti-GRK6 Antibody

Product Name

Anti-GRK6 Antibody

View all GRK6 Antibodies

SKU/Catalog Number

A03623-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GRK6 Antibody (Catalog # A03623-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

GRK6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-GRK6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03623-1)

Host

Rabbit

Contents

GRK6 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

GRK6 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human GRK6. The immunogen is located within the last 50 amino acids of GRK6.

Cross-reactivity

GRK6 antibody is human, mouse and rat reactive. Multiple isoforms of GRK6 are known to exist. This antibody is predicted to not cross-react with other members of the GRK protein family.

Reactive Species

A03623-1 is reactive to GRK6 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

66.0 kDa

Background of GRK6

The G protein–coupled receptor kinases (GRKs) are a versatile family of kinases that play a critical role in cancer metastasis through their regulation of G-protein coupled receptors (GPCRs) involved in growth factor mediated cell migration (1,2). Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state (1). GRK6 is one of 7 members of the GRK serine/threonine kinase subfamily, which has been shown to modulate the Wnt signaling pathway via phosphorylation of LRP6 (3,4), and the insulin-like growth factor signaling pathway (4). GRK6 may also play a role in immune system function (5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03623-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

GRK6 antibody can be used for detection of GRK6 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information GRK6

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