Click image to see more details
Product Info Summary
| SKU: | M01338 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GRM5/Mglur5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01338
BM4391 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GRM5/Mglur5 Rabbit Monoclonal Antibody catalog # M01338. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GRM5/Mglur5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01338)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EBH-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GRM5
Reactive Species
M01338 is reactive to GRM5 in Human, Mouse, Rat
Observed Molecular Weight
150 kDa
Calculated molecular weight
132.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01338 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: mouse brain
Validation Images & Assay Conditions
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Western blot analysis of GRM5 expression in mouse brain lysate.
Specific Publications For Anti-GRM5/Mglur5 Rabbit Monoclonal Antibody (M01338)
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Customer Q&As
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7 Customer Q&As for Anti-GRM5/Mglur5 Rabbit Monoclonal Antibody
Question
I am looking for to test anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 on rat brain for research purposes, then I may be interested in using anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
The products we sell, including anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-21
Question
Does anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 work for IF with brain?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
According to the expression profile of brain, GRM5 is highly expressed in brain. So, it is likely that anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 will work for IF with brain.
Boster Scientific Support
Answered: 2019-05-17
Question
Does anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 work on canine IF with brain?
Verified Customer
Verified customer
Asked: 2018-06-06
Answer
Our lab technicians have not tested anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 on canine. You can run a BLAST between canine and the immunogen sequence of anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-06
Question
Can you help my question with product M01338, anti-GRM5/Mglur5 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
V. Roberts
Verified customer
Asked: 2016-10-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01338 anti-GRM5/Mglur5 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-10-28
Question
Is there a BSA free version of anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 available?
F. Kulkarni
Verified customer
Asked: 2016-10-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-10-17
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338. Let me know if you need anything else.
S. Anderson
Verified customer
Asked: 2016-01-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-01-26
Question
Is this M01338 anti-GRM5/Mglur5 Rabbit Monoclonal antibody reactive to the isotypes of GRM5?
T. Moore
Verified customer
Asked: 2015-05-14
Answer
The immunogen of M01338 anti-GRM5/Mglur5 Rabbit Monoclonal antibody is A synthesized peptide derived from human GRM5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-05-14
