Product Info Summary
| SKU: | PA1590 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GST3/GST pi/GSTP1 Antibody Picoband®
SKU/Catalog Number
PA1590
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GST3/GST pi/GSTP1 Antibody catalog # PA1590. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GST3/GST pi/GSTP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1590)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human GST3/GST pi, different from the related rat and mouse sequences by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1590 is reactive to GSTP1 in Human, Mouse, Rat
Observed Molecular Weight
23 kDa
Calculated molecular weight
23.4 kDa
Background of GSTP1
Glutathione S-transferases pi, also known as GST3, present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. The GST-pi gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. The GST-pi gene is mapped to chromosome 11. Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GSTP does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1590 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Kidney Tissue, HELA Whole Cell, COLO320 Whole Cell
IHC: Human Intestinal Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-GST3/GST pi antibody, PA1590, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Click image to see more details
Anti-GST3/GST pi antibody, PA1590,Western blotting
All lanes: Anti GST3/GST pi (PA1590) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
Click image to see more details
( A ) Control ( B ) Antrochoanal polyp (ACP) patient. The expression of GSTP1 in ACP patients and controls
is not statistically different. (GSTP1; original magnification: x200). (Control; original
magnification: x150).
Index in PubMed under a CC BY license. PMID: 40443552
Specific Publications For Anti-GST3/GST pi/GSTP1 Antibody Picoband® (PA1590)
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