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Product Info Summary
| SKU: | A02461-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GSTO1 Antibody
SKU/Catalog Number
A02461-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-GSTO1 Antibody catalog # A02461-1. Tested in WB, IHC, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-GSTO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02461-1)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human GSTO1 recombinant protein (Position: G3-L241).
Reactive Species
A02461-1 is reactive to GSTO1 in Human, Mouse, Rat
Observed Molecular Weight
28 kDa
Calculated molecular weight
27.6 kDa
Background of GSTO1
This gene encodes a member of the omega class of glutathione S-transferase (GST) proteins. GSTs are involved in the metabolism of xenobiotics and carcinogens. There is evidence that the encoded protein is involved in the biotransformation of arsenic.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02461-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot (WB): 1:500-2000
Immunohistochemistry (IHC): 1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400
Enzyme linked immunosorbent assay (ELISA): 1:100-1000
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human PC-3 whole cell, human HepG2 whole cell, rat brain tissue, rat liver tissue
Validation Images & Assay Conditions
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Western blot analysis of GSTO1 using anti-GSTO1 antibody (A02461-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTO1 antigen affinity purified polyclonal antibody (A02461-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSTO1 at approximately 28 kDa. The expected band size for GSTO1 is at 28 kDa.
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