Anti-GSTT1 Antibody Picoband™

Product Name

Anti-GSTT1 Antibody Picoband™

View all GSTT1 Antibodies

SKU/Catalog Number

A01084

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GSTT1 Antibody Picoband™ catalog # A01084. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GSTT1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01084)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human GSTT1 recombinant protein (Position: L3-L231).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01084 is reactive to GSTT1 in Human, Mouse, Rat

Applications

A01084 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

27 kDa

Calculated molecular weight

27.335kDa

Background of GSTT1

GSTT1 (glutathione S-transferase theta 1), also known as GST class-theta-1, Glutathione transferase T1-1, is an enzyme that in humans is encoded by the GSTT1 gene. It is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human theta-class GST, termed GSTT1 has 239-amino acid GSTT1 protein shares 80% sequence identity with the rat homolog. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of GSTT1 using anti-GSTT1 antibody (A01084).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTT1 antigen affinity purified polyclonal antibody (Catalog # A01084) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTT1 at approximately 27KD. The expected band size for GSTT1 is at 27KD.

Gene/Protein Information For GSTT1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.5.1.18; glutathione S-transferase theta 1; glutathione S-transferase theta-1; Glutathione transferase T1-1; GST class-theta-1 GSTT1 glutathione S-transferase theta 1 glutathione S-transferase theta-1|GST class-theta-1|glutathione transferase T1-1

For more info on GSTT1, check out the GSTT1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GSTT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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