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Product Info Summary
| SKU: | EK1639 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Hyaluronidase1/HYAL1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1639
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Hyaluronidase1/HYAL1 ELISA Kit PicoKine® (96 Tests). Quantitate Human HYAL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Hyaluronidase1/HYAL1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1639)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: F22-W435
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1639 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1639 is reactive to HYAL1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1639 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of HYAL1
Hyaluronidase-1, also known as HYAL1 or LUCA1, is an enzyme that in humans is encoded by the HYAL1 gene. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1639-CAP | Anti-Human HYAL1 Pre-coated 96-well strip microplate | 1 |
| EK1639-ST | Human HYAL1 Standard | 2 vials, 10 ng/tube |
| EK1639-DA | Human HYAL1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.038 | 0.106 | 0.179 | 0.281 | 0.499 | 0.845 | 1.346 | 1.829 |
Data Example Images
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Human HYAL1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect HYAL1, Dilution ratio of 1:100, concentration in serum and plasma is around 100 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Hyaluronidase1/HYAL1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 67 | 223 | 399 | 72 | 204 | 415 |
| Standard deviation | 4.42 | 14.49 | 21.14 | 5.26 | 14.07 | 26.14 |
| CV (%) | 6.6% | 6.5% | 5.3% | 7.3% | 6.9% | 6.3% |
Specific Publications For Human Hyaluronidase1/HYAL1 ELISA Kit PicoKine® (EK1639)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human Hyaluronidase1/HYAL1 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
D. White
Verified customer
Asked: 2020-07-11
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-07-11
Question
Q: What is the optimal O.D. value for Hyaluronidase 1 ELISA kit? I used your Hyaluronidase 1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Hyaluronidase 1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-07-03
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Hyaluronidase 1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Hyaluronidase 1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-07-03
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
W. Jones
Verified customer
Asked: 2019-07-28
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-07-28
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Hyaluronidase 1 using the EK1639 Human Hyaluronidase 1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-01-16
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-12-02
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-12-02
Question
Q: if the enzyme conjugated Hyaluronidase 1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Hyaluronidase 1 antigen?
Verified Customer
Verified customer
Asked: 2018-11-20
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-11-20
Question
Q: how to analyze ELISA data? I have obtained Hyaluronidase 1 level in serum.
B. Nelson
Verified customer
Asked: 2018-09-13
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2018-09-13
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
T. Kumar
Verified customer
Asked: 2018-08-16
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2018-08-16
