Anti-GZF1 Antibody

Product Name

Anti-GZF1 Antibody

View all GZF1 Antibodies

SKU/Catalog Number

A11916

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GZF1 Antibody (Catalog # A11916). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

GZF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-GZF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11916)

Host

Rabbit

Contents

GZF1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

GZF1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GZF1. The immunogen is located within amino acids 600 - 650 of GZF1.

Cross-reactivity

GZF1 antibody is human specific. At least four isoforms of GZF1 are known to exist.

Reactive Species

A11916 is reactive to GZF1 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

80.5 kDa

Background of GZF1

The GDNF-inducible zinc finger protein 1 (GZF1) is a sequence-specific transcriptional repressor with a BTB/POZ domain and ten zinc finger motifs whose expression is required for renal branching morphogenesis during kidney development. GZF1 binds to the 5'regulatory region of the homeodomain protein HOXA10, suggesting that GZF1 may play a role in morphogenesis other than kidney development. Recent experiments have indicated that GZF1 associates with nucleolin and this association is mediated by the first four zinc finger motifs of GZF1. It is thought that Nucleolin modulates the subcellular localization of GZF1 as well as its transcriptional repressor activity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11916 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

GZF1 antibody can be used for detection of GZF1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information GZF1

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