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Product Info Summary
| SKU: | A00781-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-H-ERG/KCNH2 Antibody Picoband™
SKU/Catalog Number
A00781-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-H-ERG/KCNH2 Antibody Picoband™ catalog # A00781-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-H-ERG/KCNH2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00781-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human H-ERG/KCNH2 recombinant protein (Position: A121-V1074).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00781-3 is reactive to KCNH2 in Human, Mouse, Rat
Applications
A00781-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
130 kDa
Calculated molecular weight
126.655kDa
Background of Kv11.1
KCNH2, also known as HERG or KV11.1, encodes the pore-forming subunit of a rapidly activating-delayed rectifier potassium channel. It is mapped to 7q36.1. KCNH2 forms the major portion of one of the ion channel proteins (the 'rapid' delayed rectifier current (IKr)) that conducts potassium (K+) ions out of the muscle cells of the heart (cardiac myocytes), and this current is critical in correctly timing the return to the resting state (repolarization) of the cell membrane during the cardiac action potential. What’s more, KCNH2 channels show gating properties consistent with many of the outwardly rectifying potassium channels, but they also have an inactivation mechanism that attenuates efflux during depolarization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of H-ERG/KCNH2 using anti-H-ERG/KCNH2 antibody (A00781-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissuelysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H-ERG/KCNH2 antigen affinity purified polyclonal antibody (Catalog # A00781-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H-ERG/KCNH2 at approximately 130 kDa. The expected band size for H-ERG/KCNH2 is at 127 kDa.
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Figure 2. Flow Cytometry analysis of JK cells using anti-H-ERG/KCNH2 antibody (A00781-3).
Overlay histogram showing JK cells stained with A00781-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-H-ERG/KCNH2 Antibody (A00781-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For KCNH2 (Source: Uniprot.org, NCBI)
Gene Name
KCNH2
Full Name
Potassium voltage-gated channel subfamily H member 2
Weight
126.655kDa
Superfamily
potassium channel family
Alternative Names
Eag homolog; Eag-related protein 1; ERG; erg1; ERG-1; Ether-a-go-go-related gene potassium channel 1; ether-a-go-go-related potassium channel protein; Ether-a-go-go-related protein 1; H-ERG; HERG1; HERGhERG-1; Kv11.1; LQT2; potassium voltage-gated channel subfamily H member 2; potassium voltage-gated channel, subfamily H (eag-related), member 2; SQT1; Voltage-gated potassium channel subunit Kv11.1 KCNH2 ERG-1, ERG1, H-ERG, HERG, HERG1, Kv11.1, LQT2, SQT1 potassium voltage-gated channel subfamily H member 2 potassium voltage-gated channel subfamily H member 2|eag homolog|eag-related protein 1|ether-a-go-go-related gene potassium channel 1|ether-a-go-go-related potassium channel protein|ether-a-go-go-related protein 1|human ether-a-go-go-related|long QT syndrome type 2|potassium channel, voltage gated eag related subfamily H, member 2|potassium voltage-gated channel, subfamily H (eag-related), member 2|voltage-gated potassium channel subunit Kv11.1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCNH2, check out the KCNH2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-H-ERG/KCNH2 Antibody Picoband™ (A00781-3)
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