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Product Info Summary
| SKU: | A00241-4 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H2A.X/H2AFX Antibody
SKU/Catalog Number
A00241-4
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Histone H2A.X/H2AFX Antibody catalog # A00241-4. Tested in WB, IHC, ICC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-Histone H2A.X/H2AFX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00241-4)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human Histone H2A.X/H2AFX recombinant protein (Position: S2-S140).
Reactive Species
A00241-4 is reactive to H2AX in Human, Monkey, Mouse, Rat
Calculated molecular weight
15.1 kDa
Background of H2AX
H2A histone family member X (usually abbreviated as H2AX) is a type of histone protein from the H2A family encoded by the H2AFX gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00241-4 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-400
Immunocytochemistry/Immunofluorescence, 1:50-400
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 1:100-1000
Validation Images & Assay Conditions
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Western blot analysis of Histone H2A.X/H2AFX using anti-Histone H2A.X/H2AFX antibody (A00241-4).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse 3T3-L1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H2A.X/H2AFX antigen affinity purified polyclonal antibody (A00241-4) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Histone H2A.X/H2AFX at approximately 17 kDa. The expected band size for Histone H2A.X/H2AFX is at 15 kDa.
Specific Publications For Anti-Histone H2A.X/H2AFX Antibody (A00241-4)
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