Anti-HAAO Antibody Picoband™

Product Name

Anti-HAAO Antibody Picoband™

View all HAAO Antibodies

SKU/Catalog Number

A10482-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-HAAO Antibody Picoband™ catalog # A10482-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-HAAO Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10482-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human HAAO recombinant protein (Position: E52-G286). Human HAAO shares 86.3% and 88.5% amino acid (aa) sequence identity with mouse and rat HAAO, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A10482-1 is reactive to HAAO in Human, Rat

Applications

A10482-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

33 kDa

Calculated molecular weight

32.556kDa

Background of HAAO

3-hydroxyanthranilate 3,4-dioxygenase (EC 1.13.11.6) is an enzyme encoded by the HAAO gene. 3-Hydroxyanthranilate 3,4-dioxygenase is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is also present in low amounts in the central nervous system. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of HAAO using anti-HAAO antibody (A10482-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma paracancerous (HCCP) tissue lysates,
Lane 2: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAAO antigen affinity purified polyclonal antibody (Catalog # A10482-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAAO at approximately 33 kDa. The expected band size for HAAO is at 33 kDa.

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Figure 2. IF analysis of HAAO using anti-HAAO antibody (A10482-1).
HAAO was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HAAO Antibody (A10482-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For HAAO (Source: Uniprot.org, NCBI)

Alternative Names

3-HAO; 3-hydroxyanthranilate 3,4-dioxygenase; 3-hydroxyanthranilic acid dioxygenase; EC 1.13.11.6,3-hydroxyanthranilate oxygenase; HAD; HAO HAAO 3-HAO, HAO, VCRL1, h3HAO 3-hydroxyanthranilate 3,4-dioxygenase 3-hydroxyanthranilate 3,4-dioxygenase|3-hydroxyanthranilate oxygenase|3-hydroxyanthranilic acid dioxygenase|HAD

For more info on HAAO, check out the HAAO Infographic

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In this infographic, you will see the following information for HAAO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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