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Product Info Summary
| SKU: | M01495 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Hax1a Monoclonal Antibody [8F9G7]
SKU/Catalog Number
M01495
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hax1a Monoclonal Antibody [8F9G7] (Catalog # M01495). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Hax1a monoclonal antibody can be stored at -20°C, stable for one year.
Cite This Product
Anti-Hax1a Monoclonal Antibody [8F9G7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01495)
Host
Mouse
Contents
Hax1a Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 8F9G7
Isotype
IgG1,k
Immunogen
Mouse monoclonal Hax1a antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human Hax1a.
Reactive Species
M01495 is reactive to HAX1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
31.6 kDa
Background of HAX1
The HS-1 associated protein X-1 (Hax1) was initially identified in a yeast two-hybrid assay on the basis of its ability to bind to the hemapoietic cell-specific protein 1 (HS-1). Hax1 possesses anti-apoptotic activity and is structurally related to Bcl-2 family members, including the presence of BH1- and BH2-like domains. It has recently been shown to interact with HIV viral protein R (Vpr), a protein required for viral pathogenesis of HIV and linked to T-cell apoptosis through activation of caspases 3 and 9. Other studies indicate that Hax1-mediated processing of HtrA2 (also known as Omi) by the mitochondrial protease PARL allows survival of lymphocytes and neurons when cytokines are limiting. At least four isoforms of Hax1 are known to exist. This antibody is expected to recognize the longest isoform (Hax1a) as well as the shortest.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01495 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Hax1a antibody can be used for detection of Hax1A by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Hax1a in human brain tissue lysate with Hax1a antibody at (A) 1 and (B) 2 μg/mL.
Specific Publications For Anti-Hax1a Monoclonal Antibody [8F9G7] (M01495)
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