Product Info Summary
SKU: | A03046 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-HMG box-containing protein 1 HBP1 Antibody
SKU/Catalog Number
A03046
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-HMG box-containing protein 1 HBP1 Antibody (Catalog # A03046). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HMG box-containing protein 1 HBP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03046)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human HBP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03046 is reactive to HBP1 in Human, Mouse, Rat
Applications
A03046 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
57.645kDa
Background of HBP1
Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.
Lemercier C., Mol. Cell. Biol. 20:6627-6637(2000).
Hillier L.W., Nature 424:157-164(2003).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from COS-7 cells, using HBP1 antibody A03046.
Protein Target Info & Infographic
Gene/Protein Information For HBP1 (Source: Uniprot.org, NCBI)
Gene Name
HBP1
Full Name
HMG box-containing protein 1
Weight
57.645kDa
Alternative Names
HMG box-containing protein 1 HBP1 HMG-box transcription factor 1 HMG box-containing protein 1|high mobility group box transcription factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HBP1, check out the HBP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HMG box-containing protein 1 HBP1 Antibody (A03046)
Hello CJ!
No publications found for A03046
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-HMG box-containing protein 1 HBP1 Antibody
Question
We are currently using anti-HBP1 antibody A03046 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The anti-HBP1 antibody (A03046) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-HBP1 antibody A03046. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-15
Question
Is there a BSA free version of anti-HBP1 antibody A03046 available?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HBP1 antibody A03046 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-28
Question
Does anti-HBP1 antibody A03046 work on feline WB with liver?
T. Huang
Verified customer
Asked: 2017-01-03
Answer
Our lab technicians have not validated anti-HBP1 antibody A03046 on feline. You can run a BLAST between feline and the immunogen sequence of anti-HBP1 antibody A03046 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-03