Product Info Summary
SKU: | A02511 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HCAR2 Antibody
View all HM74A/PUMA-G/GPR109A/NIACR1 Antibodies
SKU/Catalog Number
A02511
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HCAR2 Antibody catalog # A02511. Tested in WB,IHC,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HCAR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02511)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
recombinant Protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02511 is reactive to Hcar2 in Human, Mouse, Rat
Applications
A02511 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
41.401kDa
Background of HM74A/PUMA-G/GPR109A/NIACR1
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
Patrick Most, J. Biol. Chem., Nov 2003; 278: 48404 - 48412.
Miki Okada, J. Biol. Chem., Feb 2004; 279: 4221 - 4233.
Melanie Boerries, Mol. Cell. Biol., Jun 2007; 27: 4365 - 4373.
Patrick Most, J. Clin. Invest., Dec 2004; 114: 1550 - 1563.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-HCAR2 Antibody A02511
Western blot analysis of extracts of various cell linesProtein Target Info & Infographic
Gene/Protein Information For Hcar2 (Source: Uniprot.org, NCBI)
Gene Name
Hcar2
Full Name
Hydroxycarboxylic acid receptor 2
Weight
41.401kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
GPR109A; HCA2; HCAR2; HM74A; hydroxycarboxylic acid receptor 2; Niacr1; Nicotinic Acid Receptor; PUMAG Hcar2|Gpr1, Gpr10, Gpr109a, Gpr109b, HM74, Ni, Niacr1, PUMA, PUMA-G, Pum, Pumag, mHM74b|hydroxycarboxylic acid receptor 2|hydroxycarboxylic acid receptor 2|G protein-coupled receptor 109A|G protein-coupled receptor 109B|G-protein coupled receptor 109|G-protein coupled receptor HM74|interferon-gamma inducible gene, Puma-g|niacin receptor 1|nicotinic acid receptor|putative seven transmembrane spanning receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Hcar2, check out the Hcar2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Hcar2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HCAR2 Antibody (A02511)
Hello CJ!
A02511 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The activation of retinal HCA2 receptors by systemic beta-hydroxybutyrate inhibits diabetic retinal damage through reduction of endoplasmic reticulum stress and the NLRP3 inflammasome
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-HCAR2 Antibody
Question
Has A02511 been confirmed for IHC, or if it is strictly WB?
Verified customer
Asked: 2019-08-20
Answer
The Anti-HCAR2 Antibody (A02511) was only validated for WB. We do not guarantee it will work for IHC.
Boster Scientific Support
Answered: 2019-08-21