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Product Info Summary
| SKU: | A01230-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-HDAC-5 (internal) Antibody
SKU/Catalog Number
A01230-2
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-HDAC-5 (internal) Antibody (Catalog # A01230-2). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-HDAC-5 (internal) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01230-2)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-HDAC5 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding 325-375aa of human HDAC5 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01230-2 is reactive to HDAC5 in Human
Applications
A01230-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
121.978kDa
Background of HDAC5
HDAC5 is a ubiquitous protein responsible for the deacetylation of lysine residues on the N-terminal part of the core histones. A tag for epigenetic repression, Anti-HDAC5 is an enzyme involved in transcriptional regulation, cell cycle progression and developmental events. HDAC5 is a class II histone deacetylase as defined by its similarities to other members of this class. HDAC5 is a major player in muscle differentiation, acting by repressing transcription of myocyte enhancer MEF2C. During differentiation it moves from the nucleus into the cytoplasm, allowing for myocyte enhancer factor expression. Anti-HDAC5 antibody is ideal for researchers interested in Stem Cell, Cardiovascular, or Epigenetics and Nuclear Signaling research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1: 10,000
IHC: 5 µg/mL
WB: 1ug/ml
Validation Images & Assay Conditions
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Figure 1. IHC analysis of HDAC5 using anti-HDAC5 antibody (A01230-2).
HDAC5 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HDAC5 Antibody (A01230-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For HDAC5 (Source: Uniprot.org, NCBI)
Gene Name
HDAC5
Full Name
Histone deacetylase 5
Weight
121.978kDa
Superfamily
histone deacetylase family
Alternative Names
Antigen NY-CO-9; EC 3.5.1.98; FLJ90614; HD5; histone deacetylase 5; KIAA0600; NY-CO-9 HDAC5 HD5, NY-CO-9 histone deacetylase 5 histone deacetylase 5|antigen NY-CO-9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HDAC5, check out the HDAC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HDAC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HDAC-5 (internal) Antibody (A01230-2)
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No publications found for A01230-2
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Customer Reviews
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